Special Issue on Electroporation-Based Technologies and Treatments

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Special Issue on Electroporation-Based Technologies and Treatments

By |2018-11-29T01:50:04+01:00September 28th, 2015|

Special Issue on Electroporation-Based Technologies and Treatments

The Journal of Membrane Biology

Volume 248, Issue 5, October 2015

Issue Editors: Damijan Miklavcic, Lluis M. Mir, P. Thomas Vernier

Special Issue on Electroporation-Based Technologies and Treatments

This special issue of the Journal of Membrane Biology contains reports on recent developments in the field of electroporation by participants in the International Workshop and Postgraduate Course on Electroporation-Based Technologies and Treatments held in November 2014 in Ljubljana. This was the eighth edition of what is now an annual event, first organized in 2003. The 66 participants—students, young scientists, invited lecturers, faculty members, and special guests—came from 15 countries (Algeria, Belgium, China, Croatia, France, Germany, Ireland, Israel, Italy, Lithuania, Macedonia, Netherlands, Slovenia, Spain, and United States). In addition to lectures on the physics, electrical engineering, and biology of electroporation, this year’s sessions included topical talks on electrochemical processes associated with pulsed electric field exposures, electroporation in food and biomass processing, systemic electrochemotherapy, and electrochemotherapy of colorectal liver metastases. One of the features of this week-long workshop course is the practical training offered each afternoon. Organized and led by associates of the Laboratory of Biocybernetics of the Faculty of Electrical Engineering at the University of Ljubljana, and by visiting researchers, these laboratory sessions provide a unique opportunity for hands-on experience guided by experts, with activities including gene electrotransfer, ratiometric fluorescence microscopy, microbial inactivation, electrical characterization of artificial membranes, treatment planning for electrochemotherapy, and molecular dynamics simulations. The school is now an established and unique platform for the acquisition of theoretical and practical knowledge of electroporation mechanisms and applications.

The peer-reviewed selection of articles in this issue provides a cross section of the ongoing electroporation related research being carried out by participants at the Ljubljana meeting. We are grateful to the contributors for their efforts in presenting their recent results, which will challenge readers, whether new or old in the field, to evaluate assumptions and consider new ideas, and we are especially grateful to our scientific colleagues who reviewed the manuscripts.

The workshop and postgraduate course were conducted within the scope and with the support of the European Associated Laboratory on Pulsed Electric Fields Applications in Biology and Medicine (LEA EBAM) and co-organized by COST TD1104 Action (http://www.electroporation.net/).

Finally, we acknowledge the indispensable support of the agencies, societies, and companies who sponsor the school, in particular, the Slovenian Research Agency, the Centre National de la Recherche Scientifique (CNRS), and the Bioelectrochemical Society, which have sponsored the school from its very beginning, and also IGEA (Italy), Mediline (Slovenia), Iskra Medical (Slovenia), BIA Separations (Slovenia), LTFE (Slovenia), and Betatech (France), who made it possible to increase student participation through reduced fees and lodging expenses.


This text is published as Introduction to Fifth Special Issue on Electroporation-Based Technologies and Treatments by Damijan Miklavcic, Lluis M. Mir and P. Thomas Vernier. J Membrane Biol (2015) 248:825–826
DOI 10.1007/s00232-015-9836-3

In this issue (11 articles):

  1. Editorial
    Introduction to Fifth Special Issue on Electroporation-Based Technologies and Treatments
    Damijan Miklavčič, Lluis M. Mir, P. Thomas Vernier
    Pages 825-826
    Download PDF (358KB) View Article
  2. Original Paper
    Optimization of the Electroformation of Giant Unilamellar Vesicles (GUVs) with Unsaturated Phospholipids
    Marie Breton, Mooud Amirkavei, Lluis M. Mir
    Pages 827-835
    Download PDF (899KB) View Article
  3. Original Paper
    Picosecond and Terahertz Perturbation of Interfacial Water and Electropermeabilization of Biological Membranes
    P. Thomas Vernier, Zachary A. Levine, Ming-Chak Ho…
    Pages 837-847
    Download PDF (1780KB) View Article
  4. Original Paper
    Content Delivery of Lipidic Nanovesicles in Electropermeabilized Cells
    P. Henri, R. Ospital, Justin Teissié
    Pages 849-855
    Download PDF (594KB) View Article
  5. Original Paper
    Different Cell Viability Assays Reveal Inconsistent Results After Bleomycin Electrotransfer In Vitro
    Baltramiejus Jakštys, Paulius Ruzgys…
    Pages 857-863
    Download PDF (487KB) View Article
  6. Original Paper
    Mathematical Models Describing Chinese Hamster Ovary Cell Death Due to Electroporation In Vitro
    Janja Dermol, Damijan Miklavčič
    Pages 865-881
    Download PDF (945KB) View Article
  7. Original Paper
    A Comparative Study on the Effects of Millisecond- and Microsecond-Pulsed Electric Field Treatments on the Permeabilization and Extraction of Pigments from Chlorella vulgaris
    Elisa Luengo, Juan Manuel Martínez, Mathilde Coustets…
    Pages 883-891
    Download PDF (811KB) View Article
  8. Original Paper
    Protein Extraction by Means of Electroporation from E. coli with Preserved Viability
    Sasa Haberl Meglic, Tilen Marolt, Damijan Miklavcic
    Pages 893-901
    Download PDF (942KB) View Article
  9. Original Paper
    Efficient In Vitro Electropermeabilization of Reconstructed Human Dermal Tissue
    Moinecha Madi, Marie-Pierre Rols, Laure Gibot
    Pages 903-908
    Download PDF (1159KB) View Article
  10. Original Paper
    Gene Electrotransfer of Canine Interleukin 12 into Canine Melanoma Cell Lines
    Ursa Lampreht, Urska Kamensek, Monika Stimac…
    Pages 909-917
    Download PDF (2145KB) View Article
  11. Original Paper
    Improved Specificity of Gene Electrotransfer to Skin Using pDNA Under the Control of Collagen Tissue-Specific Promoter
    Spela Kos, Natasa Tesic, Urska Kamensek, Tanja Blagus…
    Pages 919-928
    Download PDF (973KB) View Article