print small

Participating Countries:

Algeria

Argentina

Australia

Austria

Belgium

Bosnia and Herzegovina

Bulgaria

Croatia

Czech Republic

Denmark

Finland

France

FYR of Macedonia

Germany

Greece

Iceland

Ireland

Israel

Italy

Lithuania

Morocco

Netherlands

New Zealand

Poland

Portugal

Romania

Russian Federation

Serbia

Slovenia

Spain

Sweden

Switzerland

Turkey

Ukraine

United Kingdom

United States

Member area provided by LTFE
COST is supported by the EU Framework Programme Horizon 2020
This website is supported by COST

University of Zaragoza, Faculty of Veterinary

Tell your friend  | 

C/ Miguel Servet, 177
50013 Zaragoza
Spain

Contact email:

Visit the website.

Brief presentation of the institution

The laboratory of the group "New technologies of food procesing" is part of the Department of Food Science and Animal Production within the Faculty of Veterinary at the University of Zaragoza (Spain). Members of the group are involved in both research and teaching (undergraduate and graduate programs). The group has concentrated its research activities in the field of food preservation and processing by thermal and non-thermal technologies such as ultrasound, high hydrostatic pressure, pulsed electirc fields, ultraviolet light adn ionizing irradiation. Research interest is focused in critical factors affecting efficacy of technologies, kinetics and mathematical modeling of processes and mechanisms of action. The last objective of this research is to translate the basic knowledge in process innovation in order to improve food safety and quality.

Main Electroporation related topics

Microbial inactivation by PEF: Research interest covers different aspects related to microbial food safety including factors affecting microbial resistance, kinetics and mathematical modelling of microbial inactivation, sublethal injury and understanding mechanisms of microbial inactivation and resistance. The last objective in this topic is defining treatment conditions for pasteurization of heat-sensitive foods by PEF or combination of PEF with other preservation processes.
Extraction of intracellular compounds assisted by PEF: Research objective in this topic is to obtain basic knowledge in potential applications of this technology for improving extraction of intracellular compounds from plant tissues and to transfer this knowledge to different extraction processes for the food, pharmaceutical or biotechnological industry.
 

Main Electroporation related research techniques and equipment

-Batch treatment chamber to investigate electroporation and microbial inactivation by PEF at different temperatures at near-isothermal conditions
-Determination of occurrence of sublethal injury in microbial cells
-Determination of microbial electroporation by measurements of the intake/uptake of fluorescents dyes: Epifluorescence microscopy (Nikon Eclipse E400) and Espectrofluorometer (Genios, Tecan)
-Determination of electroporation of eukaryote cells by impedance measurements (DIL, Quakenbrück, Germany).
-Oscilloscopes (Tektronix, TDS 3012, TDS 220), high-voltage probe (Tektronix, P6015A), Function generator (Tektronix, 3022, AFG320)

Pulse generators:
-Exponential decay PEF generator (Input voltage: 16 kV, current intensity: 5000 A, Frequency: 100 Hz)
-Square wave PEF generator (Input voltage: 30 kV, current intensity: 200 A, Frequency: 300 Hz, Pulse width: 3 µs, Power: 6 kW).

Treatment chambers:
-Batch parallel electrode:  Area: 1 to 20 cm2. Gap: 0.25 to 2 cm. Volume: 0.5 to 40 ml.
-Continuous parallel electrode: Area: 0.63 to 2.8 cm2. Gap: 0.4 cm. Volume: 0.25 to 1.12 ml.
-Continuous collinear electrode: Diameter 0.4 cm, Gap: 0.56 cm Volume: 2 zones of 0.28 ml.
-Continuous collinear electrode for pre-industrial demonstrations: Diameter: 1 to-4 cm, Gap 1 to 4 cm. Volume: 2 zones of 12.6 to 50.24 ml
 

Research topics, techniques and equipment not related to Electroporation

-Apparatus to determine microbial and enzymatic heat resistance in the range of 50 to 160ºC
-Apparatus to determine microbial and enzymatic heat resistance to ultrasound, ultrasound under pressure (Manosonication) and combination of ultrasound under pressure and heat (Manothermosonication)
-Apparatus to determine microbial resistance to ultraviolet irradiation and combination of ultraviolet irradiation and heat.
-Apparatus to investigate ultrasound assisted extraction and Manosonication assisted extraction.
-Variable atmosphere workstation (MACS VA 500)
-HPLC (Varian ProStar)

Brief information on available facilites

- Laboratory of microbiology
- Pilot plant facilities for food processing (http://ppcta.unizar.es/ind.htm)

Willingness to accept/host visiting researchers

Publications

Projects:

  • Influence of oxidative stress on bacterial inactivation by different technologie for food preservation
    Project type: National
    Funding: Spanish ministery of education and science
  • Molecular approach to the mechanism of microbial inactivation and resistance and its application to the design of combined processes for food preservation
    Project type: National
    Funding: Spanish ministery of education and science

Members:

  • Ignacio Álvarez
    Email:

    Position: Professor
  • Beatriz Chueca
    Email:

    Position: PhD student
  • Santiago Condón
    Email:

    Position: Professor
  • Laura María Espina
    Email:

    Position: PhD student
  • Diego García
    Email:

    Position: Professor
  • Elisa Gayán
    Email:

    Position: PhD student
  • Elisa Luengo
    Email:

    Position: PhD student
  • Pilar Mañas
    Email:

    Position: Professor
  • Rafael Pagán
    Email:

    Position: Professor
  • Javier Raso
    Email:

    Position: Professor
  • María Jesús Serrano
    Email:

    Position: PhD student

Project Office

Working groups

Steering Committee

Founding members

DC Rapporteurs

Related sites: