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Participating Countries:

Algeria

Argentina

Australia

Austria

Belgium

Bosnia and Herzegovina

Bulgaria

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Czech Republic

Denmark

Finland

France

FYR of Macedonia

Germany

Greece

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Serbia

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COST is supported by the EU Framework Programme Horizon 2020
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Sofia University St. Kliment Ohridski, Biological Faculty

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8 Dragan Tzankov blvd
1164 Sofia
Bulgaria

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Brief presentation of the institution

The Department of Biophysics and Radiobiology is a part of the Biological Faculty of Sofia University "St. Kliment Ohridski" - oldest and biggest University in Bulgaria. Head of Department is Prof. Valentina Ganeva. The Department was established in 1973 and since then the main topics of investigation are the structure and the function of biological membranes. The staff of the Laboratory of Biological membranes work for more than 20 years in collaboration with the team of Prof. J. Teissie from IPBS, CNRS-Toulouse, France

Main Electroporation related topics

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Electroporation, electrotransformation, membrane electropermeabilization, electroextraction of soluble intracellular biologically active substances. Our efforts are pointed out on the electroinduced release of industrial enzymes and vitamins from different yeast strains. The last five years experiments have been performed in collaboration with Spanish company BIOMEDAL which is specialized in constructing different recombinant yeast strains expressing enzymes of industrial interest.

Main Electroporation related research techniques and equipment

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The application of high voltage pulse electric field in the range of 2 to 8 kV/cm and pulse duration of 0.5 to 1.5 ms provokes irreversible damage of cell membranes and facilitates the release of proteins. By using different postpulse incubation condition we are trying to augment the recovery of recombinant proteins and to obtain some selectivity of the release in order to facilitate the following purification process. In the laboratory we have 3 high voltage generators – Betatech (France), Cytopulse PA4000 (USA) and more powerful device for in flow electric treatment (GBS Elektroniks, Germany), equipped with devices for shape form and control of field intensity and pulse duration. We have also different chambers for in flow pulsation, some of which with incorporated thermocouple ensuring control of temperature during in flow pulsation.

Research topics, techniques and equipment not related to Electroporation

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Prompt and delayed chlorophyll fluorescence analysis with very sophisticated equipment from Hansatech (UK), oxygen evolving system in chloroplasts, free radicals processes (Lumat3, Berthold, Germany), light and fluorescent microscopes, protein and NA electrophoresis devices, fluorimeter, spectrophotometers, thermo shakers, peristaltic pumps, sterile box, small lab equipment. We can use also the apparatuses in all departments of Biological Faculty.

Brief information on available facilites

/Laboratory for bacterial cultures

Laboratory for plant tissue culture

Labotratory of Photobiophysics

Laboratory of Cell electrophoresis

Willingness to accept/host visiting researchers

Projects:

  • “Protein electroextraction coupled to direct sorption – a new route for primary recovery of intracellular bioproducts from industrial yeast” 2008-2011
    Project type: International
    Funding: 250 000 Euros

Members:

  • Boyana Angelova
    Email:

    Position: PhD student
  • Bojidar Petkov Galutzov
    Email:

    Position: Professor
  • Valentina Jordanova Ganeva
    Email:

    Position: Professor
  • Vassilii Nikolaevich Goltsev
    Email:

    Position: Professor

Project Office

Working groups

Steering Committee

Founding members

DC Rapporteurs

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