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Participating Countries:

Algeria

Argentina

Australia

Austria

Belgium

Bosnia and Herzegovina

Bulgaria

Croatia

Czech Republic

Denmark

Finland

France

FYR of Macedonia

Germany

Greece

Iceland

Ireland

Israel

Italy

Lithuania

Morocco

Netherlands

New Zealand

Poland

Portugal

Romania

Russian Federation

Serbia

Slovenia

Spain

Sweden

Switzerland

Turkey

Ukraine

United Kingdom

United States

Member area provided by LTFE
COST is supported by the EU Framework Programme Horizon 2020
This website is supported by COST

Ruđer Bošković Institute

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Bijenička 54
10000 Zagreb
Croatia

Contact email:

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Brief presentation of the institution

The Ruđer Bošković Institute (RBI, established 1950) is regarded as Croatia’s leading scientific institute in the natural and biomedical sciences as well as marine and environmental research. The primary task of the RBI is to conduct excellent basic research, with particular emphasis on complex interdisciplinary programs.  In addition to basic research, these include the development of innovative research, participation in higher education and knowledge transfer to the economy, with the goal of developing new hightech products and increasing public awareness of the importance of knowledge and science in modern society. The RBI carries out these tasks in cooperation with universities, scientific institutes and other similar institutions in Croatia and other countries.

Organizationally, the RBI consists of 12 divisions and 2 centres. Over 550 scientists and researchers in more than 80 laboratories pursuing research in theoretical and experimental physics, physics and materials chemistry, electronics, physical chemistry, organic chemistry and biochemistry, molecular biology and medicine, the sea and the environment, informational and computer sciences, laser and nuclear research and development.

Main Electroporation related topics

Currently, in the framework of COGITO bilateral project, we are studying electrotransfer of plasmid DNA into model systems (e.g. lipid vesicles and living cells) by using complementary surface methods (electrochemical and atomic force microscopy) and mathematical modelling. This would improve general knowledge on basic processes involved in active transport of therapeutic molecules through cell membrane.

Main Electroporation related research techniques and equipment

- Electrochemical adhesion-based sensor with potentiostat and Labview software for data acquisition

- Atomic force microscope (AFM)- MultiMode SPM with Nanoscope IIIa controller, Veeco Instruments

- Epifluorescent microscope, Olympus, BX51 with video camera

- Cell culture equipment, super-speed centrifuge, Herolab, HiCen 21

- Gene pulser Xcell electroporation system (BIO-RAD)

 

Research topics, techniques and equipment not related to Electroporation

- Application of atomic force microscopy for characterization of nanoparticles in marine environment
- Study of the interaction of ligand coated nanoparticles with the lipid membranes
- Study of the organization and function of the organic matter continuum in the sea
- Electrochemical characterization of organic particles in aquatic system

Brief information on available facilites

A large number of research instruments at the RBI are primarily dedicated to the Institute's research projects, but also used by other research and academic institutions in Croatia and worldwide. Some are used on a commercial basis, providing services to industry and others.

more details on:
http://www.irb.hr/eng/Research/Research-Instruments-Facilities

Willingness to accept/host visiting researchers

Publications

Projects:

  • Croatia-France Program Cogito Partners Hubert Curien, Electrotransfer of plasmid DNA in vesicles and cells by application of atomic force microscopy, electrochemical methods and mathematical modeling
    Project type: International
    Funding: CNRS France, MSES Croatia

Members:

  • Nadica Ivošević DeNardis
    Email:

    Position: Researcher
  • Jadranka Pečar-Ilić
    Email:

    Position: Researcher
  • Ivica Ružić
    Email:

    Position: Researcher
  • Suzana Šegota
    Email:

    Position: Researcher

Project Office

Working groups

Steering Committee

Founding members

DC Rapporteurs

Related sites: