print small

Participating Countries:

Algeria

Argentina

Australia

Austria

Belgium

Bosnia and Herzegovina

Bulgaria

Croatia

Czech Republic

Denmark

Finland

France

FYR of Macedonia

Germany

Greece

Iceland

Ireland

Israel

Italy

Lithuania

Morocco

Netherlands

New Zealand

Poland

Portugal

Romania

Russian Federation

Serbia

Slovenia

Spain

Sweden

Switzerland

Turkey

Ukraine

United Kingdom

United States

Member area provided by LTFE
COST is supported by the EU Framework Programme Horizon 2020
This website is supported by COST

Leibniz Institute for Plasma Science and Technology

Tell your friend  | 

Felix-Hausdorffstrasse 2
17489 Greifswald
Germany

Contact email:

Visit the website.

Brief presentation of the institution

The Leibniz Institute for Plasma Science and Technology (INP Greifswald) is the largest non-university research institute in Europe that is dedicated to research and development in the field of non-thermal plasmas. The mission of the institute is application-driven basic research from idea to prototype. Accordingly, research is conducted with a close look at industry-needs and often with industry partners.Currently two Research Divisons are focused on Materials & Energy and on Environment & Health, respectively. The priorities that are pursued in eight Research Programmes accordingly are: Surfaces and Thin Films, Catalytic Materials, Process Monitoring, Welding and Switching, Bioactive Surfaces, Plasma Medicine and Decontamination, Pollutant Degradation, and Bioelectrics.

Main Electroporation related topics

Electroporation-related research is the main topic of the Research Programme Bioelectrics. The focus of our experiments are biophysical phenomena that are instigated by the interaction of cells with short pulsed electric fields, in particular nanosecond pulsed electric field exposures, and the subsequently induced biochemical processes. As such we investigate dielectric properties of cells, membrane charging mechanisms, and transport phenomena.In a complementary effort we develop nanosecond high voltage pulse generators and delivery systems for the exposure of cells suspensions, cell monolayers, tissue samples, and for animal experiments.These systems are design with respect to individual experimental or treatment requirements.

Main Electroporation related research techniques and equipment

  • TDR spectrometer (Agilent DCA 86100D) and precission impedance analyzer (Agilent 4294A) for dielectric spectroscopy;
  • Terrahertz spectrometer (Batop Optoelectronics TDS1008);
  • Zeta-potential analyzer (Horiba SZ-100Z);
  • Inverted fluorescence microscope (Zeiss Axio Observer D1) together with miroinjection system;
  • Confocal microscope (Leica TCS SP5 II);
  • Pulsed laser imaging system (Radiant Dyes NarrowScan K, Continuum Sherline I): microscope (fluorescent) imaging with 5 ns resolution;
  • Automated patch-clamp system (Nanion Technologies Port-a-Patch);
  • Scanning electron microscope (Phenom ProX);
  • Bio atomic force microscope (JPK Instruments Nano Wizard AFM, Zeiss Axio Observer D1);
  • High voltage pulse generators (e.g. FID Technologies FPG 150-1NK, DEI PVX-4110, BTX Harvard Apparatus ECM 830): covering a pulse range from 10-ns/150-kV to 10-s/5-V);
  • High speed oscilloscopes (e.g. Tektronix DPO7204D, LeCroy WaveSurfer 64MXs-B, Tektronix TDS3054C) for high voltage pulse diagnostics.

Research topics, techniques and equipment not related to Electroporation

A second topic of the Research Programme Bioelectrics is the investigation of pulsed discharges that are generated in water by short high voltage pulses. Of particular interest are chemical reaction pathways with respect to the degradation of chemical compounds. Experiments on discharge development and water quality are conducted accordingly by fast ICCD imaging, multiple framing imaging (e.g. Specilised Imaging  SIMD-UV), high resolution spectroscopy, gas chromatography (GC-MS) and high pressure liquid chromotography (HPLC-MS).

Brief information on available facilites

  • fully equiped cell-culture laboratory with several work places;
  • fully equiped microbiolgy laboratory (class 2);
  • machine shop and electronic workshop;
  • Seminar rooms from 8-100 seats.

Willingness to accept/host visiting researchers

Project Office

Working groups

Steering Committee

Founding members

DC Rapporteurs

Related sites: